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 First stab 
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Joined: Tue Jan 27, 2009 10:52 am
Posts: 3
Post First stab
Hi, I'm new to these forums and to the biopunk movement.

There seems to be a lot of people on here who are unsure about how to get started with biohacking. To be honest, when I first found out about this I was hoping it would have been a bit better established. Lots of people have the will to start putting together their own biological systems and the material and standardization needed for it seems to be almost within reach, but not quite. It seems that sequencing DNA is impossible to do at home right now, but splicing is possible? So if we can get access to lots of different genes we can create unique pathways for desired results, right? This blot binder thing seems like a good prospect, I'd really like to get my hands on one.

I have a pretty limited background in biology (had a few courses in college) but I'm thinking about starting a small lab. The first thing I'd like to do is just make a strain of glowing bacteria just to see if I can. I know that I'll need an E. coli culture, a strand of DNA that codes for GFP, and a viral vector of some kind, right? Problem is, I'm not sure how to acquire these materials, and even if I did, how would I know if they were precisely the ones I need? I also feel like I need one of those PCR thermocyclers, but if I'm buying the DNA anyway I don't see any need to amplify it.

Anyway just wondering if I'm on the right track here


Wed Jan 28, 2009 6:41 am
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Joined: Wed Mar 19, 2008 3:19 am
Posts: 195
Location: Sweden
Post Re: First stab
Hi

I know what you mean. The situation will look very different in say 2 years I think. This is where we and tech happen to be right now. Most people don't even know this can be done.

I think the easiest way to introduce GFP to E. Coli is with the pGLO plasmid. Here is a description of the transfer:



-Splicer

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Wed Jan 28, 2009 9:16 am
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Joined: Tue Jan 27, 2009 10:52 am
Posts: 3
Post Re: First stab
this looks good, a relatively simple procedure. may not require much equipment either. I think this would be a good introduction to augmenting genomes. If nothing else I can get my feet wet in some of the basic techniques involved in plasmid transformation. It's got a couple genes that are dependent on each other but very simple overall. The next step is just to make my own plasmids.

thanks


Thu Jan 29, 2009 5:52 am
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Joined: Sat Oct 11, 2008 1:34 pm
Posts: 21
Post Re: First stab
How much does one bottle of those pGlo plasmids cost?
I registered on bio-rad.com, but still have to request an offer to get a price -.-

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Wed Mar 25, 2009 4:15 pm
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Joined: Wed Mar 19, 2008 3:19 am
Posts: 195
Location: Sweden
Post Re: First stab
I found a 32 student educational kit here for £70.

That would make it about $3.20 per unit in that case, which doesn't sound bad.

I guess it's sold exclusively in bulks though.

- Splicer

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Thu Mar 26, 2009 2:47 am
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Joined: Fri Feb 29, 2008 5:26 am
Posts: 56
Post Re: First stab
If anyone orders something from the 2 sites listed here, will they report on how successful they were? I understand that some of these companies won't sell to residential addresses. I'm hoping that is isn't the case for these sites, because they seem to have good inventory.


Sun Apr 05, 2009 2:31 pm
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Joined: Tue Sep 29, 2009 4:39 pm
Posts: 21
Location: Hungary
Post Re: First stab
No they dont unfortunately... I tried to register on the page given above to order the kit and they clearly state that they dont sell chemicals to private individuals.:( But here is an idea: If you know someone working in a university research lab he might be able to order it for you.

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Wed Sep 30, 2009 12:07 pm
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Joined: Fri Feb 29, 2008 5:26 am
Posts: 56
Post Re: First stab
You know, I have found that some of the smaller sites (the kind that target high school science teachers) are more likely to sell certain products without any big deal. Not everything, but enough to get started.

Examples:
http://www.goldbio.com
http://www.sciencestuff.com/

Also, I know that I have seen different reagents and buffers for sale on Ebay as well, definitely something to consider.


Tue Nov 10, 2009 10:04 pm
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