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 If you need help 
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Joined: Thu Jun 25, 2009 12:12 am
Posts: 22
Post If you need help
Just wanted to help any of the beginners, if you have good links please show.

Copy and past the links

Stores
http://www.invitrogen.com/site/us/en/home.html

plant culture
http://www.accessexcellence.org/LC/ST/st2bgplant.php

chicken genome map
http://www.ncbi.nlm.nih.gov/mapview/map ... taxid=9031

For studying
http://www.contexo.info/
http://askabiologist.asu.edu/expstuff/m ... index.html
http://hackteria.org/index.php/Main_Page

http://pimm.wordpress.com/2007/01/23/ho ... a-at-home/

http://www.locusplus.org.uk/biotech_hobbyistNJ.html
http://www.ebi.ac.uk/microarray/biology_intro.html
http://web.archive.org/web/200704202042 ... ahack.com/



Devices
http://www.wiley-vch.de/contents/jc_200 ... 0306_s.pdf
http://hackteria.org/index.php/Synthetic_Biology
http://makezine.com/07/fingerprinting/

hope it helps any one.


Thu Jul 16, 2009 12:55 am
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Joined: Wed Mar 19, 2008 3:19 am
Posts: 195
Location: Sweden
Post Re: If you need help
Welcome Teslaboypunk

My contribution:

Evolutionary Philosophy
http://www.evolutionary-philosophy.net/

-Splicer

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We can't stop here, this is Bat Country
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Thu Jul 16, 2009 4:28 am
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Joined: Fri Feb 29, 2008 5:26 am
Posts: 56
Post Re: If you need help
Thanks for the links, I'd hadn't come across the hacketeria site before. If you want to check out some more links, there's another thread here where others here have posted links.


Sun Jul 26, 2009 9:16 am
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Joined: Thu Jun 25, 2009 12:12 am
Posts: 22
Post Re: If you need help
Are there any safety tips for working with viruses and bacteria? Because I heard that they used gene therapy to make skin cells into stem cells. Also bacteria called agro bacterium changes plant DNA. So any links or advice is good.


Wed Aug 12, 2009 9:30 pm
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Joined: Mon Jun 01, 2009 4:34 pm
Posts: 60
Post Re: If you need help
Agrobacterium inserts plasmid DNA into plants. It won't affect you.

Viruses insert themselves into your DNA, but the worst they're likely to do is simply kill off your cells and kill you in the normal way like that, or possibly induce cancer. If you're working with bacterial cells, you'll want to use bacteriophages, which won't hurt you at all; they don't attack eukaryotic cells. I don't know if bacteriophage protein coats are sold to the general public, though; you might have to collect your own.

You shouldn't need to touch dangerous viruses at all unless you're working with animal cells or trying to engineer the virus itself.


Thu Aug 13, 2009 6:09 am
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Joined: Wed Mar 19, 2008 3:19 am
Posts: 195
Location: Sweden
Post Re: If you need help
(I think agrobacterium is suspected to infect humans too)

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Thu Aug 13, 2009 6:16 am
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Joined: Mon Jun 01, 2009 4:34 pm
Posts: 60
Post Re: If you need help
Splicer wrote:
(I think agrobacterium is suspected to infect humans too)


Huh, you're right. How strange... you'd expect more infections. It's not as if it's uncommon in nature. (Or perhaps it's only harmful in a minority of cases, or you need a really weak immune system? It does seem to favour the elderly...)


Thu Aug 13, 2009 4:58 pm
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Joined: Thu Jun 25, 2009 12:12 am
Posts: 22
Post Re: If you need help
Thank you clouded_perception, bacteriophages will be my best bet for my projects


Also I found out the first gene gun was a modded croft mans air pistol.


Thu Aug 13, 2009 7:57 pm
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Joined: Mon Jun 01, 2009 4:34 pm
Posts: 60
Post Re: If you need help
Teslaboypunk wrote:
Also I found out the first gene gun was a modded croft mans air pistol.


That's pretty awesome. It's amazing how much science can be done with "duct tape".

I can't find anybody who sells bacteriophage coats to the public but you can probably make your own. You can buy bacteriophage treatments for diseases:

http://www.phagetherapycenter.com/pii/P ... language=0

So picking the phage for whatever you're working with (E. coli, I presume? Everyone uses E. coli), culturing it and then stripping the DNA out yourself would probably be an easy way to get some. (I've never tried to do this to a bacteriophage so I can't help with info on procedure or cost.) Then you can add whatever DNA you want in the bacteria to the phage coats, and they'll take it up if it's the right size (the size is important).

Bacteriophage don't need their own DNA to function, just to reproduce. They'll inject any DNA you can convince them to take up. So if you use a mix of DNA fragments containing those of similar size to the one you want, not all of your transformed bacteria will be transformed in the way you want; you'll need a marker.

Personally I recommend trying shock before bacteriophages; it'd be way cheaper if it works. From memory, it probably should with E. coli, for instance, but you might want to get that confirmed by somebody who has more than a passing acquaintance with them and isn't typing at 3 in the morning.


Fri Aug 14, 2009 6:31 pm
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Joined: Sat Apr 04, 2009 7:51 pm
Posts: 25
Post Re: If you need help
Teslaboypunk wrote:



Why chiken?

here is human
http://www.ncbi.nlm.nih.gov/projects/ma ... taxid=9606


Thu Nov 19, 2009 11:41 pm
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