It is currently Wed Nov 22, 2017 1:38 pm




Post new topic Reply to topic  [ 3 posts ] 
 just inject DNA 
Author Message

Joined: Sat Jun 21, 2008 12:20 pm
Posts: 4
Post just inject DNA
non viral vectors, I read a paper recently, where they injected plasmids into the skin, and the reporter gene was taken in by cells.

http://eu.wiley.com/WileyCDA/WileyTitle ... 11874.html


Fri Jun 27, 2008 10:56 am
Profile E-mail

Joined: Sun Mar 23, 2008 3:00 am
Posts: 52
Post Re: just inject DNA
Was it tested on people?

I've often thought about reading up on how gene therapy actually works. The way I understand it plasmids insert their DNA at random locations in the target DNA and sometimes they get interrupted during the process resulting in an incomplete transfer. It sounds like that would suck if it's in vivo(at least in me). I wonder how they solve that. I need to go find out how gene therapy works, I realize I hardly know anything about it.

There is a discussion about HIV in the latest Futures in Biotech (FIB 32). Apparently people have successfully used the virus as a vector. HIV seems to be extremely efficiently designed and have a host of useful features(apart from the reverse transcriptase machinery).

_________________
My brother was a lawyer. All I could do was distort reality.
-Wyclef Sean


Sat Jun 28, 2008 12:21 pm
Profile E-mail

Joined: Sat Jun 21, 2008 12:20 pm
Posts: 4
Post Re: just inject DNA
it was tested on 17 melanoma patients in an proof-of-concept phase 1 study.
http://www.charite.de/forschungsbericht ... 13238.html
It lists the papers at the botom of the page.

I would not use any virus, its a non-self protein, it may interact with the iimmune system in an unhealthy way. If I flood myself with alien protein, the body may overreact.
But I am not interested to use this for therapy anyway, so all I need is getting what I want into skin cells.

This is a poster of an earlier experiment.
http://www.egms.de/en/meetings/dkk2006/06dkk589.shtml


Sat Jun 28, 2008 12:58 pm
Profile E-mail
Display posts from previous:  Sort by  
Post new topic Reply to topic  [ 3 posts ] 


Who is online

Users browsing this forum: No registered users and 0 guests


You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
cron
Powered by phpBB © phpBB Group.
Designed by Vjacheslav Trushkin for Free Forums/DivisionCore.
phpBB SEO